Protein Express
From X6AWiki
ProteinXpress is a service provided by the X6A Facility to Researchers from academia, government or industry is available to elite users (users that have experience using a beamline). X6A Elite Users can chose between remote access or staff assisted data collection.
Protein crystals can be sent either in canes or in ALS compatible pucks Automounter. All samples will be screened and data will be collected on the best samples.
Procedure for ProteinXpress:
1. Ship your samples in canes or pucks, preferably pucks (for instructions on to how use pucks see Automounter, for instructions on how to mount samples in pucks see Run Experiment Automatically (Step 1); Please contact Edwin Lazo if you would like to request pucks.
2. Use magnetic bases, screw cap base will NOT be tested (see Run Experiment Automatically (Step 1) for acceptable bases).
- If borrowing pucks from X6A, users must send a pin case so that pins may be mailed back. For pin case information [|click here]
3. Loops have to be 18 mm long (see the proper way of shipping canes section).
4. For canes a maximum of 4 canes with 5 samples on each cane (a total of 20 samples) will be accepted. If more than 20 samples pucks must be used.
In this section we only discuss the proper way of shipping samples in canes.
1. ONLY use magnetic base with 18 mm long pins.
Be consistent with the pin length: ALL PINS have to be of the same length. Always provide an empty loop. Samples mounted on screw cap bases will NOT be tested.
2. Use a loop slightly larger than the sample size and not 500 micron loop for a 50 micron sample !!!!!!
3. Label all vials properly.
4. Use cane sleeves for shipping.
5. Mount Maximum of 5 samples per cane.
6. Label all canes properly.
7. Follow the instructions to send the dry shipper :
8. Provide a spread sheet containing the following information:
Cane ID
Position (from bottom to top, so from 1 to 5 max)
Crystal ID
Protein ID
Element (Se, Hg, .....)
Type of experiment (native, MR, MAD or SAD)
Crystal size and position in the loop
If crystals were screen at home source : resolution and observations (ice rings, mosaic, multiple lattices ...)
Procedure to fill an X6A dry shipper
- fill a second container
- carefully fill the dry shipper with the second container avoiding any spills on to the neck of the dewar and on to the vacuum valve
- do not overfill the dewar
- avoid pressing the cap down to avoid liquid nitrogen spills over onto the foam or the neck or the vacuum valve on the dewar
- wait for the LN2 to evaporate naturally if the dewar is to full and then press the cap down to close the dry shipper
For any additional information or questions please contact Edwin Lazo at:
Email: elazo@bnl.gov
Phone: (631)344-7247
FAX: (631)344-3238
