Protein Express

From X6AWiki

ProteinXpress is a service provided by the X6A Facility to Researchers from academia, government or industry. X6A Elite Users can chose between remote access Remote_Access or staff assisted data collection.

Protein crystals can be sent either in canes or in ALS compatible pucks Automounter. All samples will be screened and data will be collected on the best samples.

In this section we only discuss the proper way of shipping samples in canes.

1. ONLY use magnetic base with 18 mm long pins.

Be consistent with the pin length: ALL PINS have to be of the same length. Always provide an empty loop. Samples mounted on screw cap bases will NOT be tested.

2. Use a loop slightly larger than the sample size and not 500 micron loop for a 50 micron sample !!!!!!

3. Label all vials properly.

4. Use cane sleeves for shipping.

5. Mount Maximum of 5 samples per cane.

Image:protein-express.jpg

6. Label all canes properly.

7. Follow the instructions to send the dry shipper :

Dry Shipper Shipments

8. Provide a spread sheet containing the following information:

Cane ID

Position (from bottom to top, so from 1 to 5 max)

Crystal ID

Protein ID

Element (Se, Hg, .....)

Type of experiment (native, MR, MAD or SAD)

Crystal size and position in the loop

If crystals were screen at home source : resolution and observations (ice rings, mosaic, multiple lattices ...)




Groups that want to ship samples in pucks should contact one of the beamline scientists.